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. 2023 Nov 10;13:19562. doi: 10.1038/s41598-023-46266-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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LPS induces MARCKS expression through the TLR4 dependent pathway. (A, B) WT IMMs and ΔTLR4 IMMs were treated with LPS for 6 h or left unstimulated (indicated as “unstim” in this and subsequent panels). TNF and MARCKS mRNA levels were measured using real-time PCR. Each experiment was performed in triplicate. Data are representative of two independent experiments (3 biological replicates per condition per experiment; 24 samples total) and shown as mean ± SEM: ***p < 0.005, ****p < 0.0001 (one-way ANOVA). (C) ΔTLR4 IMMs were treated with LPS for the indicated times. MARCKS protein expression was detected by western blot and beta-actin was used as a loading control. The full-length blot image is included in the Supplementary Information file, Supplementary Fig. 3. (D) Densitometric analysis of western blot results. MARCKS was normalized to beta-actin. Data are representative of 2 independent experiments, shown as mean ± SEM.