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. 2023 Nov 10;13:19562. doi: 10.1038/s41598-023-46266-x

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

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ΔMARCKS IMMs are deficient in mitochondrial respiration. (A) Illustration of oxidative phosphorylation in the mitochondrial inner membrane. (B) The general pattern of a mitochondrial stress test. (C) WT and ΔMARCKS IMMs were treated with LPS (100 ng/mL) for 6 h. Oxygen consumption rates (OCR) were measured using Seahorse with indicated inhibitors. The data were normalized to total protein. (D–G) The individual parameters for basal respiration (D), maximal respiration (E), spare respiratory capacity (F) and the ATP production (G) were obtained from the Wave software program. WT and ΔMARCKS IMMs were treated with LPS (100 ng/mL) for 6 h or left unstimulated (indicated as “unstim” in this and subsequent panels). Data are representative of 2 independent experiments (3 biological replicates per condition per experiment; 24 samples total) and shown as mean ± SEM: ***p < 0.005 **p < 0.01 *p < 0.05 (two-way ANOVA). The statistically significant differences are indicated.