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. 2023 Nov 10;14(11):730. doi: 10.1038/s41419-023-06259-0

Fig. 5. USP4 interacts with TAK1 in ESCC.

Fig. 5

A–D A Co-IP assay was used to verify the protein interaction between USP4 and TAK1. E, F At 0, 3, 6, and 9 h after CHX treatment of KYSE150 and KYSE180 cell lines, Western blotting was used to detect the levels of USP4 and TAK1 proteins to evaluate the effect of USP4 on the stability of TAK1 protein. G, H Ubiquitination of TAK1 was detected by ubiquitination assay and Western blotting. I HA-WT, K48R or K63R Ub were cotransfected with Flag-USP4 into HEK293T cells. After treatment with 20 μM MG132 for 6 h, cell lysates were subjected to a ubiquitination assay, and the ubiquitination level of TAK1 was detected by an anti-HA antibody. *P < 0.05, **P < 0.01, ***P < 0.001.