Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Nov 11;14(11):733. doi: 10.1038/s41419-023-06264-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — A, B The mRNA and protein expressions of MELK in normal human hepatocytes (MIHA) and different HCC cell lines: HepG 2, Hep 3B, Huh7, and PLC/PRF/5. After MELK expression was silenced or overexpressed in Huh7 cells: MELK expression was detected by qPCR (C), WB (D) and immunofluorescence (E); Cell proliferation was evaluated with CCK-8 assay (F), colony formation assay (G) and EdU assay (H); Stemness was evaluated with the 3D sphere formation assay (I); Cell migration and invasion were investigated with transwell assays (J, K); Biomarkers of EMT and stemness were detected by WB (L, M). After MELK knockdown in the HCC cell line Hep3B and the normal human hepatocytes MIHA: MELK expression was detected by qPCR (N, R); Cell proliferation was evaluated with CCK-8 assay (O, S) and EdU assay (P); Cell migration and invasion were investigated with transwell assays (Q, T). Data were shown as mean ± SEM. n.s. represents not significant. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 as calculated by the one-way or two-way ANOVA. Scale bar: 10 µm.