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. 2023 Nov 10;13:19617. doi: 10.1038/s41598-023-46759-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Incubation of the 30 min pellicle-coated enamel samples for 1 min in Coke Zero and subsequent oral exposure for a further 89 min resulted in partial protein desorption of the (a) bovine and (d) human permanent samples, whereas the equine samples showed only reduced thickness (b). The human primary samples showed acid-induced dissolution of the pellicle, resulting in a subsurface pellicle (c). The former enamel site is marked with an asterisk as it was removed during the preparation process. Original magnification: 49,000-fold.