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Resistance of ureC mutants to urea in stationary phase. Wild-type cells of Synechococcus sp. strain PCC 7002 (strain PR6000) (lane A, control) and cells of ureC mutant strains PR6091 (lane B) and PR6092 (lane C) were grown in medium A+ supplemented with 100 mM urea and 5 μM NiSO4 for 5 days. The mutant cells did not die and were not bleached, even after prolonged incubation (up to 11 days), although wild-type cells were killed and bleached after 3 days when grown in this medium.
