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Purification of the extracellular lecithinase from a phase I variant culture of X. nematophilus F1
| Prepn | Vol (ml) | Amt of total protein (mg) | Total activitya | Sp actb | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Culture supernatant | 100 | 145 | 580,000 | 4 | 100 | 1 |
| SP chromatography | 3 | 0.36 | 324,000 | 900 | 56 | 225 |
| C18 chromatography | 0.2 | 0.008 | 11,200 | 1,400 | 2 | 350 |
Expressed as speed of diffusion (in millimeters per 24 h).
Expressed as speed of diffusion (in millimeters per 24 h) per microgram of protein.
