TABLE 4.
Substrate specificity of the purified lecithinase on agarose-lipid layersa
| Substrate of lipase | Results |
|---|---|
| Egg yolk lecithin (60% PC)b | +c |
| Phosphatidylcholine (99%)d | + |
| Lyso-phosphatidylcholine (99%)d | + |
| Phosphatidylinositol (99%)d | + |
| Lyso-phosphatidylinositol (99%)d | + |
| Phosphatidylethanolamine (99%)d | − |
| Sphingomyelin (99%)d | + |
| Diacylglycerol | − |
| Phosphatidic acid (99%)d | + |
| Triolein | − |
| p-Nitrophenylphosphorylcholine | − |
| p-Nitrophenylpalmitate | − |
a
As measured by the formation of a white halo of precipitation surrounding a hole in an agarose-lipid layer filled with 3 μl of the purified lecithinase.
b
Containing 60% phosphatidylcholine.
c
+, strong precipitation; −, no precipitation.
d
Percent purity.