TABLE 3.
Possible virulence factors of T. pallidum (30) and corresponding gene expression levels in treponemes isolated from rabbit testes
| ORF or protein category | cDNA/DNA signal ratio | Coding strand | Putative gene function | cDNA/DNA signal ratio normalized to that of TP0426
|
|
|---|---|---|---|---|---|
| DNA microarray | Real-time PCR | ||||
| Tpr proteins | |||||
| TP0897 | 1.1 | 0 | Tpr protein K (tprK) | ||
| TP0610 | 0.92 | 1 | Tpr protein H (tprH) | 0.71 | 0.74 |
| TP1031 | 0.76 | 1 | Tpr protein L (tprL) | 0.58 | 3.44 |
| TP0621 | 0.75 | 0 | Tpr protein J (tprJ) | 0.58 | 0.15 |
| TP0317 | 0.74 | 0 | Tpr protein G (tprG) | 0.57 | 0.88 |
| TP0117 | 0.74 | 0 | Tpr protein C (tprC) | ||
| TP0131 | 0.70 | 0 | Tpr protein D (tprD) | ||
| TP0313 | 0.70 | 1 | Tpr protein E (tprE) | 0.54 | 0.20 |
| TP0316 | 0.68 | 0 | Tpr protein F, authentic frame shift (tprF) | 0.52 | 0.69 |
| TP0620 | 0.65 | 0 | Tpr protein I (tprI) | 0.50 | 0.21 |
| TP0011 | 0.39 | 1 | Tpr protein B (tprB) | ||
| TP0009 | 0.32 | 0 | Tpr protein A, authentic frame shift (tprA) | 0.24 | 0.13 |
| Hemolysins | |||||
| TP1037 | 1.1 | 0 | Hemolysin III (hlyIII) | 0.83 | 0.49 |
| TP0936 | 0.78 | 0 | Putative hemolysin | 0.60 | 0.74 |
| TP0649 | 0.57 | 0 | Hemolysin (tlyC) | ||
| TP0027 | 0.30 | 1 | Putative hemolysin | ||
| TP0028 | 0.23 | 1 | Putative hemolysin | 0.18 | 0.09 |
| Regulators | |||||
| TP0981 | 0.98 | 1 | Putative sensory transduction histidine kinase | ||
| TP0454 | 0.94 | 0 | Hypothetical protein (regA) | ||
| TP0038 | 0.79 | 0 | Regulatory protein (pfoS/R) | ||
| TP0980 | 0.73 | 1 | Histidine phosphokinase/phosphatase (ntrB) | ||
| TP0877 | 0.41 | 1 | Conserved hypothetical protein (regC) | ||
| TP0516 | 0.19 | 0 | Virulence factor (mviN) | 0.15 | 0.15 |
| TP0520 | 0.16 | 0 | Sensory transduction histidine kinase, authentic frame shift | ||
| TP0519 | 0.15 | 0 | Response regulatory protein (atoC) | 0.11 | 0.08 |
| Polysaccharide biosynthesis | |||||
| TP0562 | 1.5 | 1 | Spore coat polysaccharide biosynthesis protein (spsE) | ||
| TP0440 | 0.89 | 1 | Putative spore coat polysaccharide biosynthesis protein | ||
| TP0283 | 0.79 | 1 | Lipopolysaccharide core biosynthesis protein (kdtB) | ||
| TP0107 | 0.44 | 0 | LicC protein (licC) | ||
| TP0078 | 0.33 | 1 | Spore coat polysaccharide biosynthesis protein (spsC) | ||
| TP0077 | 0.31 | 1 | Capsular polysaccharide biosynthesis protein (cap5D) | ||
| TP0288 | 0.25 | 1 | Spore coat polysaccharide biosynthesis protein (spsF) | ||
| Potential membrane or surface-exposed proteins | |||||
| TP0574 | 17.7 | 0 | Carboxypeptidase, 47 kDa | ||
| TP0971 | 12.6 | 0 | Membrane antigen, pathogen specific (tpd) | 9.7 | 12.4 |
| TP0319 | 9.7 | 1 | Membrane lipoprotein (tmpC) | 7.5 | 12.8 |
| TP0768 | 6.7 | 1 | Membrane protein (tmpA) | 5.2 | 8.3 |
| TP0171 | 5.9 | 1 | Lipoprotein, 15 kDa (tpp15) | ||
| TP0769 | 5.2 | 1 | Outer membrane protein (tmpB) | 4.0 | 2.2 |
| TP0435 | 5.1 | 0 | Lipoprotein, 17 kDa (tpp17) | 3.9 | 10.5 |
| TP0486 | 3.3 | 0 | Antigen, p83/100 | ||
| TP0298 | 3.3 | 1 | Exported protein (tpn38b) | ||
| TP0470 | 3.1 | 0 | Conserved hypothetical protein | ||
| TP0729 | 2.9 | 0 | Treponemal aqueous protein (tap1) | ||
| TP0006 | 2.5 | 1 | Tp75 protein | ||
| TP0993 | 2.5 | 0 | Putative rare lipoprotein A | ||
| TP0225 | 2.3 | 1 | Leucine-rich repeat protein TpLRR | ||
| TP0702 | 2.2 | 0 | Conserved hypothetical protein | ||
| TP0020 | 2.2 | 1 | 76K protein | ||
| TP1038 | 2.1 | 0 | Bacterioferrin (tpF1) | 1.7 | 3.4 |
| TP0821 | 1.5 | 0 | Lipoprotein (tpn32) | ||
| TP0571 | 1.3 | 0 | Tp70 protein | ||
| TP0163 | 1.1 | 1 | ABC transporter, periplasmic binding protein (troA) | ||
| TP1016 | 1.0 | 0 | Basic membrane protein (tpn39b) | ||
| TP0957 | 0.78 | 1 | Tp33 protein | ||
| TP0624 | 0.66 | 1 | Conserved hypothetical protein | ||
| TP0819 | 0.65 | 0 | Conserved hypothetical protein | ||
| TP0326 | 0.62 | 1 | Outer membrane protein | ||
| TP0989 | 0.58 | 0 | P26 | ||
| TP0567 | 0.54 | 0 | Conserved hypothetical protein | ||
| TP0327 | 0.52 | 1 | Cationic outer membrane protein (ompH) | ||
| TP0796 | 0.46 | 0 | Conserved hypothetical protein | ||
| TP0292 | 0.42 | 1 | Outer membrane protein (tpn50) | ||
| TP0034 | 0.36 | 0 | ABC transporter, periplasmic binding protein | ||
| Miscellaneous functions | |||||
| TP0835 | 0.51 | 0 | Putative ankyrin | ||
| TP0680 | 0.50 | 0 | o-Sialoglycoprotein endopeptidase (gcp) | ||
| TP0502 | 0.42 | 1 | Hypothetical protein | ||
| TP0580 | 0.19 | 1 | Conserved hypothetical integral membrane protein | ||