TABLE 3.
Genes upregulated by two different garlic extracts (G1 and G6) and 4-NPO
| PA genea | Gene | Regulation | Descriptionb | Fold Changec
|
||
|---|---|---|---|---|---|---|
| G1 | G6 | 4-NPO | ||||
| PA0047 | Hypothetical protein | 3.0 | 2.6 | 5.8 | ||
| PA0083 | Conserved hypothetical protein | 2.5 | 3.3 | 5.8 | ||
| PA0085 | Conserved hypothetical protein | 3.1 | 3.6 | 8.8 | ||
| PA0197 | Hypothetical protein | 8.1 | −1.6 | −1.9 | ||
| PA0198 | exbB1 | Transport protein ExbB | 6.2 | −2.1 | −2.5 | |
| PA1556 | Probable cytochrome c oxidase subunit | 5.4 | 1.8 | 11.0 | ||
| PA1557 | Probable cytochrome oxidase subunit (cbb3 type) | 3.3 | 1.6 | 8.3 | ||
| PA1673 | Hypothetical protein | 2.0 | 1.8 | 18.1 | ||
| PA1715 | pscB | Type III export apparatus protein | 7.4 | 5.4 | 4.6 | |
| PA1718 | pscE | Type III export protein PscE | 4.4 | 2.7 | 6.5 | |
| PA1746 | Hypothetical protein | 1.1 | −1.1 | 7.1 | ||
| PA2285 | Hypothetical protein | −1.1 | −1.1 | 5.4 | ||
| PA2310 | Hypothetical protein | 5.9 | −3.2 | 1.0 | ||
| PA3283 | Conserved hypothetical protein | 2.4 | 2.4 | 25.4 | ||
| PA3284 | Hypothetical protein | 2.0 | 2.0 | 12.1 | ||
| PA3441 | las + rhl | Probable molybdopterin binding protein | 5.9 | −2.5 | −2.5 | |
| PA3442 | Probable ATP-binding component of ABC transporter | 9.2 | −1.9 | −2.5 | ||
| PA3444 | Conserved hypothetical protein | 8.3 | 1.1 | −1.1 | ||
| PA3445 | Conserved hypothetical protein | 5.3 | −1.1 | 1.3 | ||
| PA3570 | mmsA | Methylmalonate-semialdehyde dehydrogenase | 7.6 | 2.4 | −3.0 | |
| PA3720 | Hypothetical protein | 10.2 | 3.2 | 1.1 | ||
| PA3935 | tauD | Murine dioxygenase | 8.4 | −1.3 | 1.4 | |
| PA3938 | Probable periplasmic taurine binding protein precursor | 6.2 | −1.3 | −1.2 | ||
| PA4067 | oprG | Outer membrane protein OprG precursor | 2.3 | 1.6 | 5.9 | |
| PA4191 | Probable iron-ascorbate oxidoreductase | 5.9 | 1.2 | 1.0 | ||
| PA4195 | Probable binding protein component of ABC transporter | 7.1 | 1.7 | −1.1 | ||
| PA4710 | phuR | Hemoglobin uptake outer membrane receptor PhuR precursor | 4.6 | 5.5 | 2.7 | |
| PA5027 | Hypothetical protein | 1.3 | 1.8 | 6.3 | ||
| PA5054 | hslU | Heat shock protein HslU | 4.4 | 5.4 | 2.3 | |
| PA5083 | Conserved hypothetical protein | 5.8 | 2.3 | 1.2 | ||
| PA5475 | Hypothetical protein | −1.0 | −1.2 | 5.4 | ||
a
Gene marked with boldface were found to be QS regulated (25).
b
Description from the Pseudomonas genome project (http://www.pseudomonas.com).
c
Values are fold changes in hybridization signal comparing a treated and untreated planktonic culture at OD600 = 2.0; data in boldface indicate a change of fivefold or higher. LasR-RhlR regulation is defined by Hentzer et al. (25).