TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant features | Source |
|---|---|---|
| Strains | ||
| E. coli K-12 | ||
| TOP10 | High-efficiency transformation | Invitrogen, Carlsbad, Calif. |
| S17 | Tra functions of IncP plasmids integrated into chromosome | 30 |
| BL21(DE3)pLysS | lacl, PlacUV5-T7 RNA polymerase, ΔompT, Δlon | Novagen, San Diego, Calif. |
| B. pertussis | ||
| BP536 | Tohama I, Nalr Strr | 40 |
| TM1627 | Tohama I, Nalr Strr ΔrisA | This study |
| TM1793 | Tohama I, Nalr, Strr, ΔbvgR | This study |
| Bp106 | Clinical isolate | CBER Bordetella Collectiona |
| Bp188 | Clinical isolate | CBER Bordetella Collection |
| Bp509 | Clinical isolate | CBER Bordetella Collection |
| Bp10536 | Clinical isolate | CBER Bordetella Collection |
| Plasmids | ||
| pSS1577 | Apr KanrrpsL oriT cos | 35 |
| pSS2809 | Apr GmroriT; B. pertussis DNA, E. coli lac operon | 8 |
| pCR2.1-TOPO | TA cloning vector | Invitrogen |
| pET22b | Lacl-repressed T7 promoter, 6His tag | Novagen |
| pTM119 | Apr KmrrpsL oriT cos bvgR | This study |
| pTM120 | Apr KmrrpsL oriT cos ΔbvgR | This study |
| pTM255 | pCR2.1-TOPO-risA | This study |
| pTM266 | pCR2.1-TOPO-ΔrisA | This study |
| pTM268 | Apr GmrrpsL oriT cos ΔrisA | This study |
| pTM275 | pCR2.1-TOPO-risA | This study |
| pTM276 | pET22b-risA | This study |
a
CBER, Center for Biologics Evaluation and Research.