TABLE 2.

Substrate specificities of PAP^a

Substrate	Relative activity (%)
AMP	100
GMP	10
UMP	0.13
CMP	0.09
IMP	2.2
dAMP	18
dGMP	2.6
dCMP	0.008
TMP	0.012

^aReaction mixtures (each, 50 μl) containing purified PAP, 50 mM Tris-HCl buffer (pH 8.0), 100 mM MgCl₂, 10 mM poly(P), and 5 mM NMP or dNMP were incubated at 37°C. The amount of the product ribonucleoside diphosphate or deoxyribonucleoside diphosphate was measured by HPLC (9).