TABLE 5.
Effect of deletion of the BaeR-binding site sequence
| Gene | Fold increasea
|
||
|---|---|---|---|
| Strain NKE11 (wild type, pUCbaeR) | Strain NKE122 (ΔBaeR binding motif for acrD, pUCbaeR) | Strain NKE124 (ΔBaeR binding motif for mdtA, pUCbaeR) | |
| mdtA (yegM) | 490 | 510 | 1.1 |
| mdtB (yegN) | 280 | 300 | 0.6 |
| mdtC (yegO) | 190 | 190 | 0.4 |
| mdtD (yegB) | 110 | 120 | 1.1 |
| baeS | 120 | 130 | 0.6 |
| acrD | 35 | 1.7 | 38 |
| tolC | 12 | 13 | 12 |
| spy | 640 | 600 | 590 |
| baeR | 15,000 | 17,000 | 11,000 |
a
The values in boldface type indicate increases of more than twofold compared to the expression levels of the genes in NKE10 (W3110/pUC118).