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Sucrose gradient analysis of 70S ribosomes and their subunits from wild-type and nus mutant strains
| Strain or genotype | Peak areaa
|
% Relative to 70S
|
30S/50S ratio | |||
|---|---|---|---|---|---|---|
| 30S | 50S | 70S | 30S | 50S | ||
| MC4100 | 1.7 | 1.8 | 30.7 | 5.7 | 5.9 | 1 |
| nusB::IS10 | 4.8 | 1.8 | 37.7 | 12.7 | 4.8 | 2.5 |
| ΔnusA::cat rhoE134D | 5.9 | 2.1 | 38.8 | 14.4 | 5.1 | 3 |
Relative molar ratio.
