TABLE 3.
Sulfatase and sulfoglycosidase activities in cell extracts of selected anaerobes able to react with 6-SO3-β-GlcNAc-1-pNP
| Bacteriuma | Cell fraction | Activity at pH 5.5
|
||
|---|---|---|---|---|
| Uninhibited activity (nmol/min/mg) | 6-SO2NH2-β-GlcNAc-1-Me-inhibited activity (nmol/min/mg)b | % Inhibition | ||
| Prevotella strain RS2 | Extract | 253.3 | 191.5 | 24.4 |
| Membrane | 353.1 | 224.5 | 36.4 | |
| P. melaninogenica ATCC 25845 | Extract | 10.5 | 4.8 | 54.3 |
| Membrane | 16.8 | 3.9 | 76.8 | |
| B. fragilis ATCC 25285 | Extract | 22.0 | 21.5 | 2.3 |
| Membrane | 121.6 | 108.7 | 10.6 | |
| B. thetaiotaomicron ATCC 29148 | Extract | 6.9 | 5.5 | 20.3 |
| Membrane | 19.5 | 10.0 | 48.7 | |
a
Soluble extracts and membrane preparations from the following isolates were unable to release pNP from 6-SO3-β-GlcNAc-1-pNP: Prevotella buccae ATCC 33577, Prevotella corporis ATCC 33547, Prevotella intermedia ATCC 25611 and WR 2122, Prevotella oralis ATCC 33269 and UI 0279, Prevotella bivia US 4025, Peptostreptococcus anaerobius ATCC 27337, and Peptostreptococcus asaccharolyticus ATCC 14963.
b
Incubation of 0.5 mM 6-SO2-β-GlcNAc-1-Me with enzyme (16 h at 0°C) completely inhibited N-acetylglucosamine-6-sulfatase (MdsA) activity but resulted in only 20 to 40% inhibition of SGL from Prevotella strain RS2.