TABLE 3.

Specific bonds between BlaI and DNA

Protein	Atom 1	DNAa	Atom 2	Distance	Commentsb
Ser9	OG	Gua211	O2P	3.18	H.B., helix α1-TACA/TGTA
Met10	N	Gua211	O1P	3.29	H.B., helix α1-TACA/TGTA
Ala11	N	Gua211	O1P	2.91	H.B., helix α1-TACA/TGTA
Asn28	ND2	Ade216+	O1P	3.18	H.B., helix α2-dyad strand
Ser41	OG	Ade213	O2P	3.08	H.B., helix α3-TACA/TGTA
Lys43	NZ	Thy201*	O4	2.61	H.B., helix α3-opposite distal strand
Thr44	OG1	Ade213	O2P	2.97	H.B., helix α3-TACA/TGTA
Arg46	NH1	Thy201*	O1P	2.74	S.B., helix α3-opposite distal strand
Thr47	OG1	Ade213	N6	2.85	H.B., helix α3-TACA/TGTA
Arg51	NH1	Gua211	N7	2.83	H.B., helix α3-TACA/TGTA
Arg51	NH2	Gua211	O6	2.87	H.B., helix α3-TACA/TGTA
Arg60	NH1	Ade202*	O2P	3.14	S.B., strand β2-opposite distal strand
Arg60	NH2	Ade202*	O1P	2.93	S.B., strand β2-opposite distal strand
Asn65	ND2	Thy204+*	O1P	2.73	H.B., wing-opposite dyad strand
Tyr67	N	Thy201*	O2P	3.06	H.B., strand β3-opposite distal strand

^aThe interactions are centered at the DNA strand that contains the TGTA motif, whereas * indicates the opposite strand that contains the TACA motif. In the distal region, the protein also interacts with the dyad-related DNA strands, indicated by ⁺ and ⁺*.

^bS.B. and H.B., salt bridges and hydrogen bonds, respectively.