FIG. 2.
Rotavirus RNA detection by a seminested RT-PCR after infection of CaCo-2 cells with different inocula of the Itor P13 strain and cell sorting. Lanes: 1, mock-infected cells from cursor A; 2, mock-infected cells from cursor B; 3, mock-infected cells from the rest of the population; 4, cells from cursor A after infection with 0.2 MPNCU; 5, cells from cursor B after infection with 0.2 MPNCU; 6, cells from the rest of the population after infection with 0.2 MPNCU; 7, cells from cursor A after infection with 2 MPNCU; 8, cells from cursor B after infection with 2 MPNCU; 9, cells from the rest of the population after infection with 2 MPNCU; 10, cells from cursor A after infection with 200 MPNCU; 11, cells from cursor B after infection with 200 MPNCU; 12, cells from the rest of the population after infection with 200 MPNCU; M, molecular size marker; −, negative control RT-PCR; +, positive control. Cursors A and B are defined in the text. Numbers on the left and right indicate base pairs.