TABLE 5.
Organism | Mean ± SD
|
|
---|---|---|
Flux into PP pathway (%)a | Catabolic PP pathway flux (%)b | |
Z. mobilis | 0 ± 0 | 0 ± 0 |
P. fluorescens | 10 ± 1 | −2 ± 1c |
S. melioti | 4 ± 1 | −2 ± 1 |
A. tumefaciens | 6 ± 2 | −1 ± 2 |
Paracoccus versutus | 0 ± 2 | −4 ± 2 |
R. sphaeroides | 0 ± 5 | −7 ± 5 |
E. coli | 21 ± 4 | 12 ± 4 |
B. subtilis | 38 ± 1 | 27 ± 1 |
Carbon flux into the PP pathway was calculated as the flux through 6-P-gluconate dehydrogenase divided by the total carbon uptake rate.
For the catabolic PP pathway flux, the CO2 production and precursor flux of erythrose-4-P and ribulose-5-P into biomass were subtracted from those of 6-P-gluconate dehydrogenase.
Negative values indicate reverse flux into the PP pathway from fructose-6-P and/or glyceraldehyde-3-P.