FIG. 4.

Quantification of promoter activities by low-resolution S1 protection assay. The promoters preceding the coding sequences listed at the left were examined for their activities in the wild type (WT) and in the S. coelicolor A3(2) litS (ΔlitS) and litR (ΔlitR) mutants. RNA was isolated from cells cultured for 2 days under dark (−) or light (+) conditions. For crtY, litQ, litA, and litB, the signals derived from the readthrough transcripts (RT) are also shown. Transcription of hrdB was analyzed to confirm the quality and quantity of RNA.