FIG. 6.
In vitro runoff transcription by EσLitS. (A) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the σLitS protein expressed and purified by use of an E. coli host-vector system. (B) Polyacrylamide gel electrophoresis of σLitS-specific transcripts. The indicated amounts of an E. coli RNA polymerase core enzyme (E) and the recombinant σLitS protein were added to the reaction mixture with the promoter DNA fragments to generate transcripts specific for crtE (PcrtE) and crtY (PcrtY). Arrowheads indicate the specific transcripts. nt, nucleotides.