TABLE 1.

Oligonucleotide primers used in this study

Primer	Sequence (5′→3′)a	Restriction enzyme	Position (nucleotides)b
PEF	5′-AGCTGCTGGCCGGCAATCAGC		173554-173574
PER	5′-TCGACCGCGTTCGCCGCATCG		173886-173866
PERH	5′-ACCATCGGCGGCAGTCCAGG		173823-173804
PYF	5′-TACGTGTGCGGAGACCACCGG		182413-182393
PYR	5′-ACACTCTTCGGTTCCACGCTTC		182194-182215
PYRH	5′-TGCCACCGGGTCACGCACAC		182178-182197
PQF	5′-TGACCCTGGGCCCTGGATGG		183896-183877
PQR	5′-TGATGCCCAGGGGCAAAGG		183587-183605
PQRH	5′-TGCGAGTGCGGGGAAACG		183675-183692
PRF	5′-TCGTGTACGCGTGTTCATGC		184975-184956
PRR	5′-ACCTCGCCGGTGGTCAATCC		184718-184737
PRRH	5′-TCGTCGACCGGCTCGTCG		184757-184774
PSF	5′-ACTATACGACGCACAAACGACG		184804-184825
PSR	5′-TTCCTCCTGCCGTGCGTCGTACG		184952-184930
PSRH	5′-ACGTCGCCGGTCTACCCTGC		184901-184882
PAF	5′-TTCTACGAGGACCTGACGCAGG		185426-185447
PAR	5′-TTGGCGAAGACGTCGACCGTCG		185835-185814
PBF	5′-ACCAGACTCTGGCTCTGCCCG		186213-186233
PBR	5′-TCGCCACTGGCGTGACCGAGTGC		186541-186519
PhrdF	5′-CGGCCGCAAGGTACGAGTTGATGA		78385-78408
PhrdR	5′-GCCATGACAGAGACGGACTCGGCG		78726-78703
aph	5′-TGATATTGCTGAAGAGCTTGG		2228-2248
rSF	5′-GGAACGCATATGAACACCCGTACCCGCCCGAC	NdeI	184949-184980
rSR	5′-GGCCAAGCTTGACACACGTGTC	HindIII	185573-185552
DcrtF	5′-CTGGAAGCTTTACTGGTACT	HindIII	173081-173099
DcrtMR	5′-ATCAGATCTTCGTGGACCAGAGC	Bg/II	174132-174110
DcrtMF	5′-TTTAGATCTCGACGTCTCCTTCC	Bg/II	178146-178168
DcrtR	5′-CGCGGAATTCTGCTGATGTTCC	EcoRI	179422-179400
DRF	5′-CGTCGAATTCCTGCCGCCGAAG	EcoRI	186262-186241
DRMR	5′-TACGAGATCTATGCCCACTATAC	Bg/II	184787-184810
DRMF	5′-CCCGAGATCTGTTGTCGTCC	Bg/II	183799-183780
DRR	5′-GCACAAGCTTCAGGATGGCTCCG	HindIII	182651-182672
DSF	5′-CCCGTCCCCGTACGACGCACGGCAGGAGGAACGGGCATGATTCCGGGGATCCGTCGACC		184922-184960
DSR	5′-GTGGATGCGCCCCGGGGCGTCTCTGCTCCGGCCCGGTCATGTAGGCTGGAGCTGCTTC		185602-185564
DBF	5′-CCGGGAATTCACGAGGTACTC	EcoRI	185336-185356
DBMR	5′-GTGAAGATCTGCGCCCTCCTG	Bg/II	186491-186471
DBMF	5′-GAACAGATCTCCGAGGTCCG	Bg/II	186881-186900
DBR	5′-GAGGAAGCTTAGTACGTCCTCC	HindIII	188594-188573
DSABF	5′-CATAAAGCTTCTCACTCGACG	HindIII	183557-183577
DSABMR	5′-TACGAGATCTTCAGCCCGTTCC	Bg/II	184970-184952
HRF	5′-GTGGGCATGCACGCGTACACG	SphI	184805-184783
HRR	5′-GACAAGATCTCACGGGTACACC	Bg/II	183784-183805
HSF	5′-ACGGGCATGCACACGCGTAC	SphI	184952-184971
HSR	5′-TGGGAGATCTCGAAGGTGTTTCG	Bg/II	185640-185618

^aRestriction sites are underlined.

^bAll position numbers except those for primer aph correspond to the numbering in the S. coelicolor genomic sequence (http://www.sanger.ac.uk/Projects/S_coelicolor). For aph, the numbering corresponds to the description given by Beck et al.(4).