FIG. 2.

DCs phagocytose pathogenic E. coli in a DC-SIGN-independent manner. Four pathogenic E. coli strains were examined for phagocytosis by DCs alone or in the presence of anti-DC-SIGN or mannan. DCs were incubated for 2.5 h with these E. coli strains, and the internalization of E. coli by DCs was measured by killing the extracellular bacteria with gentamicin (100 μg/ml [final concentration]) (A) and confirmed by electron microscopy (B), showing the phagocytosed UPEC. The number of phagocytosed bacteria was determined by counting CFU recovered after gentamicin treatment.