TABLE 4.
Nucleotide pools in wild-type strain CCM 1904 and isogenic derivatives impaired in pyrimidine metabolism
| Strain | Genotype | Generation time (min)a | Nucleotide pool size (nmol [mg (dry wt)]−1)
|
|||
|---|---|---|---|---|---|---|
| UTP | CTP | GTP | ATP | |||
| CCM 1904 | Wild type | 103 ± 6 | 2.7 ± 0.4 | 0.9 ± 0.2 | 2.2 ± 0.4 | 6.2 ± 0.7 |
| FB335 | ΔcarAB | 113 ± 4 | 2.0 ± 0.2 | 0.6 ± 0.0 | 2.0 ± 0.3 | 5.9 ± 1.4 |
| AE1026 | pyrR1 | 107 ± 5 | 2.9 ± 0.5 | 0.9 ± 0.1 | 2.0 ± 0.4 | 5.6 ± 0.2 |
| AE1023 | at2 mutated | 106 ± 11 | 0.6 ± 0.2 | 0.4 ± 0.1 | 3.3 ± 0.4 | 7.3 ± 1.4 |
| U25 | ΔcarAB pyrR1 | 120 ± 10 | 5.9 ± 1.7 | 1.3 ± 0.5 | 1.6 ± 0.6 | 5.7 ± 0.7 |
| U33 | ΔcarAB at2 | 115 ± 5 | 2.9 ± 0.0 | 0.9 ± 0.1 | 2.7 ± 0.3 | 7.5 ± 0.0 |
a
In defined DLP medium in the presence of purines (adenine guanine, hypoxanthine, and xanthine) at final concentrations of 83, 50, 33, and 5 μg/ml, respectively.