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. 2005 Mar;187(6):2038–2049. doi: 10.1128/JB.187.6.2038-2049.2005

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FIG. 2. — (A) DNA-binding assay with purified CBP-CpxR and the promoter region of csgD. ³²P-end-labeled wild-type csgD promoter (D1D2 probe) was incubated without protein (lane 1) or with 0.48 μM (lane 2), 0.96 μM (lane 3), 1.44 μM (lane 4), 1.92 μM (lane 5), 2.4 μM (lane 6), or 2.88 μM (lane 7) CBP-CpxR. ³²P-end-labeled deleted csgD promoter (D1D3 probe) was incubated without protein (lane 8) or with 1.92 μM (lane 9) CBP-CpxR. (B) Alignment of six putative CpxR binding sites. Scores are relative to the CpxR consensus defined by De Wulf (18). Site positions are relative to the transcriptional start site of csgD. CpxR binding sites are shown in Fig. 3.