FIG. 3.

Recombinant expression of GGR homologues. Lanes 1, 7, 13, and 19, molecular standard marker; lanes 2 to 6, precipitated fractions after the cell disruption of E. coli BL21(DE3)/pET3a-AF0464, BL21(DE3)/pET3a-AF1637, BL21(DE3)/pET3a-AF1023, BL21(DE3)/pET3a-AF0648, and BL21(DE3)/pET3a, respectively; lanes 8 to 12, crude extracts from E. coli strains are shown above, in the same order; lanes 14 to 18, heat-treated enzyme from each strain, in the same order. Asterisks indicate the bands of highly expressed recombinant enzymes, whose molecular masses, as estimated from their mobility, correspond well to those calculated from their amino acid sequences. Double asterisks indicate a band that would arise from the partial degradation of the recombinant protein.