FIG. 1.

Antisense construct to spoIIE. The spoIIE antisense construct consists of oligonucleotide spoasastop (upper DNA strand) and spoasbtm (lower DNA strand). The single underlined GATC forms the 5′ BamHI “sticky” end. The black-shaded region includes 38 bases of the spoIIE open reading frame followed by 16 bases of the upstream leader sequence, shown shaded in dark gray. The bold region forms the 17-base rho-independent terminator region from an antisense RNA targeted against the glnA gene, found naturally in Clostridium saccharobutylicum NCP262 (10).