TABLE 1.
Bacterial strains and plasmidsa
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| C. acetobutylicum ATCC 824 | Wild type | ATCC |
| C. acetobutylicum SKO1 | Δspo0A MLSr | 18 |
| E. coli DH10β | mcrA ΔmcrBC recA1 Strr | NEB |
| Plasmids | ||
| pCATP | MLSr OriII ColElori catP | 35 |
| pCATspo | MLSr OriII ColElori catP, spoIIE promoter | This study |
| pSC12lacZ | Cmr OriII ColElori lacZ′ | 43 |
| pHT3 | Apr MLSr ColElori repL lacZ | 7, 39 |
| pThilac | Thir OriII ColElori lacZ | This study |
| pTLspo | Thir OriII ColElori lacZ, spoIIE promoter | This study |
| pIMP1 | Apr MLSr ColElori repL | 23 |
| pMSpo | Apr MLSr ColElori repL spoIIE | This study |
| pMSpoD | Apr MLSr ColElori repL spoIIE′ | This study |
| pSOS94 | ptb promoter, Apr MLSr ColElori repL | 38 |
| pASsos | ptb promoter, Apr MLSr ColElori repL | This study |
| pASspo | ptb promoter, Apr MLSr ColElori repL | This study |
spo0A, deletion of spo0A; MLSr, macrolide-lincosamide-streptogramin B resistant; mcrA ΔmcrBC, methylcytosine-specific restriction system abolished; recAl, homologous recombination abolished; Strr, streptomycin resistant; OriII repL, gram-positive origin of replication; ColEl, gram-negative origin of replication; catP, chloramphenicol acetyltransferase open reading frame; Cmr, chloramphenicol resistant; lacZ′, truncated, nonfunctional copy of lacZ; Apr, ampicillin resistant; lacZ, functional, promoterless β-galactosidase gene; Thir, thiamphenicol/chloramphenicol resistant due to functional copy of catP with a complete promoter; spoIIE, intact copy of spoIIE including promoter; spoIIE′, spoIIE with partial deletion of open reading frame. ATCC, American Type Culture Collection, Manassas, VA.; NEB, New England Biolabs, Beverly, Mass.