TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| R. sphaeroides | ||
| 2.4.1 | Wild type | Laboratory collection |
| APP11 | 2.4.1 appA::Tpr | 16 |
| PPS2-4 | 2.4.1 ppsR (Cys251-Ser, Cys424-Ser) | This study |
| PRRA1 | 2.4.1 prrA::Tpr | 11 |
| RPS1 | 2.4.1 prrA::TprppsR::Kmr | This study |
| P. denitrificans ATCC 17741 | Wild type | American Type Culture Collection |
| E. coli | ||
| DH5α phe | Strain used for cloning | 11 |
| S17-1 | Tra+ strain used for plasmid mobilization | 37 |
| Plasmids | ||
| pRK415 | Tcr IncP, vector | 23 |
| pPNs | pRK415::ppsR | 15 |
| pLX1 | IncQ, promoterless lacZYA:: ΩSmr-Spr | 15 |
| pLXhemC | pLX1 containing hemC::lacZYA | This study |
| pLXhemE | pLX1 containing hemE::lacZYA | This study |
| p714SmH::Kmr::mob | Suicide mobilizable plasmid, ppsR::ΩKmr | 16 |