FIG. 5.
Dose-dependent induction of S motility in DK12601. Strain DK12601 is DK10410::PpilA-lacO-RBS-pilA lacI. Strains DK1622 (wild type), DK10410 (ΔpilA), and DK12600 (DK10410::PpilA-lacO-RBS-pilA) were included as positive and negative controls. A total of 2.5 × 107 cells were spotted on 0.5× CTT-0.4% agar plates and incubated at 32°C. The concentration of IPTG used (millimolar) is indicated in the top right corner of each panel. The swarming was inspected after 48 h. The results are representative of the results of three independent experiments.