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The ColV-resistant phenotype of the TnMK mutant is the direct consequence of the sdaC mutation. Portions (5 μl) of either undiluted or 10-fold-serial-diluted ColV solution prepared from the cytoplasmic fraction of the tatC mutant expressing RR-ColV were loaded on the soft agar containing MC4100A, TnMK/pUC19, TnMK/pUSCB, or TnMK/pUSC prepared as described in Fig. 1.
