TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli DH5α | supE44 Δlac U169 (φ80 lacZM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 24 |
| E. chrysanthemi | ||
| 3937 | Wild-type strain | 30 |
| BT123 | Δ(phoP)::Tn7 Camr, derivative of 3937 | This work |
| BT124 | Δ(phoO)::Tn7 Camr, derivative of 3937 | This work |
| Plasmids | ||
| pGEM T-easy | Ampr | Promega |
| pBluescript II SK(−) | Ampr | Stratagene |
| pB113 | pGEM carrying 3937 phoP and phoQ genes | This work |
| pB114 | pBluescript II carrying 3937 phoP mutant | This work |
| pB115 | pBluescript II carrying 3937 phoQ mutant | This work |