FIG. 1.
Protein expression of VirA truncation mutants. (A) Schematic representation of VirA constructs. The plasmids expressing VirA constructs are listed on the left; in parentheses are the corresponding plasmids of VirA constructs with a G665D mutation in the kinase domain. Open arrow, virA promoter; solid arrow, PN25 promoter; white box, His6 tag. (B) Western blot analysis of VirA constructs. Clarified lysates from bacteria carrying indicated plasmids were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in 10% Bis-Tris NuPage (Invitrogen) gels followed by Western blot analysis using anti-RGS-His monoclonal antibody.