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pH and sugar responses of VirA and ChvE allele products. PvirB-lacZ expression by DL8/pRG109 containing pRG169 with wild-type VirA and PN25-chvE(T211M) at pH 5.5 (Δ), pH 5.5 with 14 mM glucose (▴), pH 7.0 ([squo]), and pH 7.0 with 14 mM glucose (▪), in terms of β-galactosidase activity calculated as Miller units (A) and expressed as percentages of the maximal activity (B). (C) PvirB-lacZ expression by wild-type VirA and VirA with aa 242 through 257 deleted (Δ242-257) with 200 μM AS.
