TABLE 1.
Step | Activitya (U) | Protein (mg) | Sp. act. (U/mg) | Purification (fold) | Recovery (%) |
---|---|---|---|---|---|
Cell extract | 28,700 | 2,600 | 11 | 1 | 100 |
DEAE | 14,500 | 495 | 29 | 2.7 | 51 |
Phenyl sepharose | 11,500 | 27 | 419 | 38 | 40 |
Hydroxyapatite | 7,051 | 8.7 | 810 | 74 | 25 |
Superdex 200 | 8,045 | 4.3 | 1,900 | 170 | 28 |
Activity was measured at 100°C by using Lys-pNA (10 mM) as the substrate.