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. 2005 Mar;187(6):2077–2083. doi: 10.1128/JB.187.6.2077-2083.2005

TABLE 1.

Purification of lysine aminopeptidase from P. furiosus

Step Activitya (U) Protein (mg) Sp. act. (U/mg) Purification (fold) Recovery (%)
Cell extract 28,700 2,600 11 1 100
DEAE 14,500 495 29 2.7 51
Phenyl sepharose 11,500 27 419 38 40
Hydroxyapatite 7,051 8.7 810 74 25
Superdex 200 8,045 4.3 1,900 170 28
a

Activity was measured at 100°C by using Lys-pNA (10 mM) as the substrate.