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Kinetic parameters for substrates of P. furiosus lysine aminopeptidase
| KAP | Substratea | Km (mM) | Vmax (μmol/min/mg) | kcatb (s−1) | kcat/Km (mM−1 s−1) |
|---|---|---|---|---|---|
| n-KAP | Lys-pNA | 4.6 | 2,900 | 1,260 | 274 |
| Lys-Gly-Gly | 1.8 | 677 | 305 | 169 | |
| r-KAP | Lys-pNA | 4.0 | 2,870 | 1,840 | 460 |
| Lys-Gly-Gly | 2.7 | 2,300 | 1,460 | 540 | |
| Arg-pNA | 1.4 | 1,200 | 769 | 550 |
All assays were carried out at 100°C in 50 mM MOPS, pH 8.0.
Based on a molecular mass of 38.2 kDa.
