TABLE 2.
Primers used in this study
| Primer | Target fragment | Sequence | Reference |
|---|---|---|---|
| psboA1 | PsboA | 5′-CCTCATAAAAAGCATTTCCT-3′ | This study |
| psboA2 | PsboA | 5′-AATTGAATCCTCCCTTTTTT-3′ | This study |
| psunA1 | PsunA | 5′-ATCCATAATAGTCAATTTTA-3′ | This study |
| psunA2 | PsunA | 5′-TTGTAAAACCTCCCCATTTG-3′ | This study |
| pyjcN-F | PyjcN | 5′-CTTTATTATGTAGTGGCTGCA-3′ | This study |
| pyjcN-R | PyjcN | 5′-TCATTTTTTCCTCCGATACCTT-3′ | This study |
| pK-F | PcomK | 5′-AATCTATCGACATATCCTGCAA-3′ | This study |
| pKplusR-R | PcomK | 5′-TTATACTAATAATCTATCATCTGTTT-3′ | This study |
| rokint1 | rok CSa | 5′-CGGGATCCCGCTTGCGGTTAGAA-3′ | This study |
| rokint2 | rok CSa | 5′-GGAATTCCGAAGCTCCGAGCTCT-3′ | This study |
| psdpF | PsdpA | 5′-CCCAAGCTTAGTTCCCAAATTCAATTCTG-3′ | This study |
| psdpR | PsdpA | 5′-GGAATTCTAATAGGAAACATATAGTCATTAC-3′ | This study |
| pyydF-F | PyydF | 5′-ATATTGATGTTTTACTTTTCAT-3′ | This study |
| pyydF-R | PyydF | 5′-CATATTATCCCTCCTCCT-3′ | This study |
| pyybN-FL | PyybN | 5′-CATGCAATTTAGTGATCCAA-3′ | This study |
| pyybN-R | PyybN | 5′-CATAATTTTACAATCCTTTCA-3′ | This study |
| pKOH-R | PcomK | 5′-TTCTGACTCATATTATGGCCT-3′ | This study |
| pykuWpa | Prok | 5′-CGGGATCCGCTTCTCTTTCATTAAACAT-3′ | 18 |
| prokps | Prok | 5′-CGGAATTCGATGTTTTTCCTCAATTTTAG-3′ | 18 |
| pcomG1 | PcomG | 5′-CCGGAATTCATGGTGACCATGTCTGCT-3′ | 33 |
| pcomG2 | PcomG | 5′-CGCGGATCCCTCTCCTTTCAACGC-3′ | 33 |
| pskfA-F | PskfA | 5′-CAAGCCGTACGAACGGACTG-3′ | This study |
| pskfA-R | PskfA | 5′-TGGATACGACCTCTTTGCCC-3′ | This study |
| pyydH-F | PyydH | 5′-GAAAAGTTAAATTCCAATTTGC-3′ | This study |
| pyydH-R | PyydH | 5′-TCATATTTTCCATCTTCATACTT-3′ | This study |
| pbhlA-F | PbhlA | 5′-GGCACAGTTAAGCTTGG-3′ | This study |
| pbhlA-R | PbhlA | 5′-GTTCACCACCTTAGTACC-3′ | This study |
| pykuJ-F | PykuJ | 5′-TAATTATGTGCATGACATTCAAAAAAAG-3′ | This study |
| pykuJ-R | PykuJ | 5′-TGCAGCCGTGTGATGATACC-3′ | This study |
| pyxaJ-F | PyxaJ | 5′-TACATGCAATATGGTATGGTG-3′ | This study |
| pyxaJ-R | PyxaJ | 5′-GTCATGATCGCCACGCTATT-3′ | This study |
| sunT-F | sunTb | 5′-GCCAAGCTTTGGGGATAAGGAAGGCT-3′ | This study |
| sunT-R | sunTb | 5′-CGGGATCCACCCACAACGAACAAGGA-3′ | This study |
| yxaJ-F | yxaJb | 5′-GCCAAGCTTAGCTGGCATGTGGTCA-3′ | This study |
| yxaJ-R | yxab | 5′-CGGGATCCGCCACAAACCAAATGACG-3′ | This study |
| yybM-F | yybMb | 5′-GCCAAGCTTTCTGGCACTTCCGT-3′ | This study |
| yybM-R | yybMb | 5′-CGGGATCCAAGGCTGTTATCAGGGA-3′ | This study |
| yydl-F | yydlb | 5′-GCCAAGCTTAGCAAAAGAATCGGCAG-3′ | This study |
| yydl-R | yydlb | 5′-CGGGATCCATCTTCTCGGGGTTCTC-3′ | This study |
| yjcN-F | yjcNb | 5′-CCCAAGCTTCTCCTGTTGTCTTTACAGCTTCTTCGG-3′ | This study |
| yjcN-R | yjcNb | 5′-CGCGGATCCCCCTGTGCCACAACTTACTTCGTATTC-3′ | This study |
| sdpA-F | sdpAb | 5′-CCCAAGCTTGAGGTTGAGCAGGACTACTATC-3′ | This study |
| sdpA-R | sdpAb | 5′-CGCGGATCCCTATTGCCTGAACTCTTCCTTC-3′ | This study |
a
Coding sequence.
b
The target sequences from this genes was inserted into pMUTIN2 to create a fusion to lacZ.