TABLE 2.
Consensus sequences of putative regulatory motifs found in the upstream regions of genes up-regulated in lactose-, starch-, and guar gum-grown cells relative to those in glucose-grown cells
| Consensusa in genes up-regulated by the indicated sugar | Locus | Function and/or identity of corresponding peptideb | Distance(s) from −10 box or start codon (nt) |
|---|---|---|---|
| Lactose | |||
| TTTCGGGAAA | TM0060 | Oligopeptide ABC transporter; PBP | 377 and 187 |
| CT | |||
| A | |||
| TM0070 | Endo-1,4-β-xylanase, XynB | 172, 128, and 103 | |
| TM0309 | Oligopeptide ABC transporter; PBP | 261 and 43 | |
| TM1204 | Maltose ABC transporter; PBP; MalE1 | 398 and 110 | |
| TM1839 | Maltose ABC transporter; PBP; MalE2 | 419 | |
| Starch 1 | |||
| TGTGATAAAAT | TM1069 | Transcriptional regulator; DeoR family | 26c |
| GTC | |||
| T | TM1839 | Maltose ABC transporter; PBP; MalE2 | 63c |
| TM1840 | α-Amylase, AmyA | 238 and 54c | |
| Starch 2 | |||
| TCCACCTGGA | TM1069 | Transcriptional regulator; DeoR family | 101 |
| ATGG | |||
| TA | |||
| TM1839 | Maltose ABC transporter; PBP; MalE2 | 285 | |
| TM1840 | α-Amylase, AmyA | 332, 293, and 92 | |
| TM1845 | Pullulanase, PulA | 199, 187, and 49 | |
| Guar gum | |||
| CCTTTGAGGAG | TM1204 | Maltose ABC transporter; PBP; MalE1 | 358 |
| GGGATC | |||
| AAC T | |||
| TM1227 | Endo-1,4-β-mannosidase | 296, 263, 211, and 142 | |
| TM1624 | β-Mannosidase | 125 and 6 |
a
Nucleotides in the consensus sequences are present in at least 70% of the aligned sequences. Nucleotides below the line are alternative nucleotides at the indicated positions in the consensus. Underlining indicates potential palindromes.
b
PBP, periplasmic substrate binding protein.
c
Distance is relative to the start codon of the corresponding gene since the −10 box is part of those motifs.