TABLE 1.

Histidine-tagged Dec protein on virions binds nickel agarose

Fraction	P22	P22 + Deca
Input phageb	100	100
Unbound phagec	60	35
Phage eluted by imidazoled	≤0.4	33

^aC-terminally histidine tagged (see the text).

^bDefined as 10070. 10¹⁰ phage (about 2 μg) in a solution containing 50 mM NaPO₄ buffer (pH = 8.0), 300 mM NaCl, 1 mM MgCl₂, and 10 mM imidazole, shaken gently with 0.5 ml nickel-nitrilotriacetic acid agarose (Qiagen) for 3 h at 4°C.

^cSame buffer conditions as described in footnote b; sum of three washes by pelleting and resuspension in 1 ml of fresh buffer.

^dSame buffer conditions as described in footnote b with 250 mM imidazole; sum of three washes by pelleting and resuspension in 0.5 ml of fresh buffer.