FIG. 1.

Standard curves using Western blotting and specific antisera to detect denatured, pure TRAP and AT proteins. Dilutions of purified standard proteins (TRAP and AT) were used for Western blotting and immunodetection with TRAP- and AT-specific antibodies. Each sample was assayed in duplicate, and the background value was used to calculate the value with no protein added (A). Quantification of TRAP and AT was performed as described in Materials and Methods. Standard curves were generated by using dilutions of purified standard proteins TRAP (B) and AT (C).