Lack of cross-reaction of TRAP and AT antisera. Western bolt analysis using anti-TRAP antiserum (A) and anti-AT antiserum (B), respectively. (A) Lanes 1 to 3: 4, 6, and 8 ng of AT protein, respectively; lane 4: 6 ng of TRAP protein. (B) Lanes 1 to 3: 0.4, 0.6, and 0.8 ng of TRAP protein, respectively; lane 4: 0.6 ng of AT protein. The positions of molecular mass markers (in kilodaltons [kd]) are shown to the sides of the blots.