Skip to main content
. Author manuscript; available in PMC: 2023 Nov 11.
Published in final edited form as: ACS Chem Biol. 2018 Jul 11;13(8):2347–2358. doi: 10.1021/acschembio.8b00568

Figure 3: Binding of PTH(1–34) to the extracellular domain (ECD) of PTH1R.

Figure 3:

(A) Construction of MBP-PTH1R-ECD. (B) SDS-PAGE gel of MBP-PTH1R-ECD shows Lane 1, markers; lane 2, purified MBP-PTH1R-ECD (62 kD) (C) Binding of PTH(1–34) to MBP-PTH1R-ECD measured by fluorescence polarization. (Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA). Each curve shows the average of three fluorescence polarization experiments.