Skip to main content
. Author manuscript; available in PMC: 2023 Nov 11.
Published in final edited form as: ACS Chem Biol. 2018 Jul 11;13(8):2347–2358. doi: 10.1021/acschembio.8b00568

Figure 4: Specific Ca2+ effect on PTH(1–34) binding to PTH1R-ND.

Figure 4:

Titration of PTH(1–34)-FAM at 50 nM with PTH1R-ND in Mg2+-depleted buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, and 100 μM EDTA) at various concentration of (A) Mg2+ and (B) Ca2+. Unless specified, each curve shows the average of at least three replicates. The 10 mM Mg2+, 40 mM Mg2+ and 10 mM Ca2+ titrations show a single experiment.