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. Author manuscript; available in PMC: 2023 Nov 11.
Published in final edited form as: ACS Chem Biol. 2018 Jul 11;13(8):2347–2358. doi: 10.1021/acschembio.8b00568

Table 1:

Binding affinity of the tested PTH1R ligands

Total Charges KD (nM) KD (0 mM) / KD (15 mM)
Peptide + / − (net charge) 0 mM Ca2+ 15 mM Ca2+
PTH(1–34) 7 / 4 (+3) 1001 ± 41 204 ± 17** 4.9 ± 0.5
PTHrP(1–36) 10 / 5 (+5) 568 ± 37 495 ± 31 * 1.1 ± 0.1
PTH(1–34)E19AE22A 7 / 2 (+5) 224 ± 18 82 ± 6 ** 2.7 ± 0.3
PTH(1–14)PTHrP(15–36) 9/4(+4) 114 ± 5 76 ± 4 1.5 ± 0.1
PTHrP(1–14)PTH(15–34) 9 / 5(+4) No binding No binding NA
*

p<0.05 and

**

p<0.001 indicate significant difference in the fitted KD values for the tested ligand in the presence and absence of 15 mM Ca2+. The ratio KD(0 mM)/KD(15 mM) for PTH(1–34) is significantly different from the same ratio for all of the other tested ligands p < 0.0001.