FIG. 3.

Visual estimation of Vero cell cytotoxicity. Wells 1 to 5 were inoculated with growth medium; well 6 was inoculated with sewage that had been centrifuged, filtered, and DNase treated; wells 7, 8, 9, and 10 were inoculated with 10-fold dilutions of a crude supernatant of E. coli O157:H7 ATCC 43888 infected with 10 ml of sewage that had been centrifuged, filtered, and DNase treated. Wells 11, 12, 13, 14, and 15 were like wells 6, 7, 8, 9, and 10, respectively, but with the addition of anti-Stx 2 subunit A MAb 11E10.