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. 2004 Nov 16;7(1):R82–R92. doi: 10.1186/bcr954

Table 1.

Hybridisation experiments

RNA no. Microarray hybridisations
1 BCAR3 (R/BCS) BCAR3 (E2 6 h) BCAR3 (R/BCS) ZR-75-1 (E2 6 h) ZR-75-1 (E2 7 d) ZR-75-1 (E2 7 d) ZR-75-1 (E2 7 d) ZR-75-1 (E2 7 d)
2 BCAR1 (R/BCS) BCAR3 (R/BCS) ZR-75-1 (R/BCS) ZR-75-1 (R/BCS) ZR-75-1 (R/BCS) BCAR1 (R/BCS) BCAR3 (R/BCS) ZR/EGFR (EGF 7 d)

RNA samples were prepared from cell lines pretreated for 5 days in RPMI 1640 medium supplemented with 10% bovine calf serum (R/BCS) and subsequently cultured for the indicated durations in R/BCS medium supplemented with oestrogen (E2), epidermal growth factor (EGF) or R/BCS medium without further additions. All hybridisations were performed in duplicate with swapping of the dyes.