Table 1.
RNA no. | Microarray hybridisations | |||||||
1 | BCAR3 (R/BCS) | BCAR3 (E2 6 h) | BCAR3 (R/BCS) | ZR-75-1 (E2 6 h) | ZR-75-1 (E2 7 d) | ZR-75-1 (E2 7 d) | ZR-75-1 (E2 7 d) | ZR-75-1 (E2 7 d) |
2 | BCAR1 (R/BCS) | BCAR3 (R/BCS) | ZR-75-1 (R/BCS) | ZR-75-1 (R/BCS) | ZR-75-1 (R/BCS) | BCAR1 (R/BCS) | BCAR3 (R/BCS) | ZR/EGFR (EGF 7 d) |
RNA samples were prepared from cell lines pretreated for 5 days in RPMI 1640 medium supplemented with 10% bovine calf serum (R/BCS) and subsequently cultured for the indicated durations in R/BCS medium supplemented with oestrogen (E2), epidermal growth factor (EGF) or R/BCS medium without further additions. All hybridisations were performed in duplicate with swapping of the dyes.