Table 2.
Primer information
| Gene | Sequence 5'→3' | |
| HPRT1 | F | TATTGTAATGACCAGTCAACAG |
| R | GGTCCTTTTCACCAGCAAG | |
| HMBS (= PBGD) | F | CATGTCTGGTAACGGCAATG |
| R | GTACGAGGCTTTCAATGTTG | |
| IGFBP5 | F | GGGTTTGCCTCAACGAAAAG |
| R | TTTCTGCGGTCCTTCTTCAC | |
| ESR1 (ERα) | F | GAGCACCCAGGGAAGCTAC |
| R | CATCAGGTGGATCAAAGTGTC | |
| CSTD (cathepsin D) | F | CACGGGCTCCTCCAACCT |
| R | GGACTTGTCGCTGTTGTACTTGTG | |
| TFF1 (PS2) | F | ATGGCCACCATGGAGAAC |
| R | TTCACACTCCTCTTCTGG | |
| MYC | F | GAGCCCCTGGTGCTCCAT |
| R | CGATTTCTTCCTCATCTTCTTGTTC | |
| ESR2 (ERβ) | F | TCAGCCTGTTCGACCAAGTG |
| R | GGCCTTGACACAGAGATATTC | |
| PGR | F | CAAGTTAGCCAAGAAGAGTTC |
| R | ACTTCGTAGCCCTTCCAAAG | |
| ERBB2 (HER2/Neu) | F | GTCTACAAGGGCATCTGGAT |
| R | GTGGATGTCAGGCAGATGC | |
| BCAR3 | F | GCGGTGGAACTGAAGGATTC |
| R | TGGCAGTTTGGGTGTACTGG | |
| BCAR1 | F | CTGCCCAGGATATTTACCAG |
| R | CGTCATACACCTCCAGCAAC | |
| EGFR | F | CGGGACATAGTCAGCAGTG |
| R | GCTGGGCACAGATGATTTTG | |
| Gene | Product code | |
| CDKN1A | HS00355782_m1 | |
| PGK1 | Hs99999906_m1 | |
| IL1R1 | Hs00168392_m1 | |
| CSTA | Hs00193257_m1 | |
| TFF3 | Hs00173625_m1 | |
| FMOD | Hs00157619_m1 | |
| PDZK1 | Hs00420042_m1 | |
| APOD | Hs00155794_m1 | |
| MGP | Hs00179899_m1 | |
| PSAT1 | Hs00253548_m1 | |
F, forward; R, reverse. The gene names are defined in Additional file 3.