Table 3.
Infectivity assay of viral infectious constructs on cucurbitaceous plants.
| Host | Inoculum | Latent period (in dpi) | Symptom appearance | Number of symptomatic plants/inoculated plants | PCR positive plants/INOCULATED plants | Disease development and virus detection (by PCR) in % |
|---|---|---|---|---|---|---|
| Ash gourd (cv. Suruchi) | A + B | 23 | Mosaic, vein banding | 16/20 | 16/20 | 80 |
| A + B + β | 21 | Mosaic, vein banding, stunting | 20/20 | 20/20 | 100 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Squash (cv. Green star) | A + B | 20 | Mosaic, yellow patches | 20/20 | 20/20 | 100 |
| A + B + β | 20 | Mosaic, downward leaf curling with puckering, stunting, yellow patches | 20/20 | 20/20 | 100 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Pumpkin (cv. Co2) | A + B | 25 | Mild mosaic, mild yellowing | 9/20 | 9/20 | 45 |
| A + B + β | 23 | Mosaic, yellowing with dark green patches, stunting | 14/20 | 14/20 | 70 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Snake gourd (cv. Co2) | A + B | 22 | Mosaic, yellowing with dark green patches` | 12/20 | 12/20 | 60 |
| A + B + β | 21 | Mosaic, yellowing with dark green patches, stunting | 16/20 | 16/20 | 80 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Ridge gourd (cv. CoH1) | A + B | 28 | Mild mosaic, mild yellow patches | 8/20 | 8/20 | 40 |
| A + B + β | 25 | Mild mosaic, mild yellow patches | 12/20 | 12/20 | 60 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Bitter gourd (cv. Co1) | A + B | 25 | Mild mosaic, mild leaf mottling | 6/20 | 6/20 | 30 |
| A + B + β | 22 | Mild leaf curling, stunting | 17/20 | 17/20 | 85 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Muskmelon (cv. Dulce F1) | A + B | 25 | Mosaic, vein banding | 10/20 | 8/20 | 50 |
| A + B + β | 23 | Mosaic, vein banding, Downward leaf curling, stunting | 15/20 | 15/20 | 75 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Cucumber (cv. Nazia F1) | A + B | 25 | Vein banding, yellowing | 12/20 | 12/20 | 60 |
| A + B + β | 23 | Vein banding, yellowing | 13/20 | 13/20 | 65 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Watermelon (cv. F1 555) | A + B | 21 | Mosaic, leaf mottling, yellowing | 12/20 | 12/20 | 60 |
| A + B + β | 20 | Mosaic, leaf mottling, yellowing, stunting | 17/20 | 17/20 | 85 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/20 | 0/20 | 0 | |
| Bottle gourd (cv. Co1) | A + B | 28 | Mild mosaic | 2/20 | 2/20 | 10 |
| A + B + β | 24 | Mosaic, yellow patches | 9/20 | 9/20 | 45 | |
| A | - | - | 0/20 | 0/20 | 0 | |
| Mock inoculated control | - | - | 0/2 | 0/20 | 0 |