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. 2004 Nov 24;7(1):R101–R112. doi: 10.1186/bcr958

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Copyright © 2004 Zivadinovic et al., licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is cited.

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Verification of the utility of the crystal violet (CV) assay for measuring cell number. (a) Different numbers of MCF-7 cells enriched for membrane estrogen receptor-α (mER^high) and MCF-7 cells depleted for membrane estrogen receptor-α (mER^low; 1000–7000/well) were plated, fixed with 2% paraformaldehyde/0.1% glutaraldehyde, and then quantified via the CV assay at 590 nm absorbance. (b) Different numbers of mER^highcells were assessed in parallel with CV and MTT assays to demonstrate a linear correlation between these assays. The values are means for 28 samples ± standard error.