Fig. 4.
In vivo models for assessing tumor penetration. (A) Schematic illustration for applying different dyes to visualize blood vessels in a tumor-bearing mouse. (B) In vivo MRI scan images of tumors. (C) (a) NIR-II imaging of mouse blood vessels at 10 min after intravenous administration of a SQ1 nanoprobe. (b, c) Zoomed-in NIR-II images for two specified regions of interest (ROI 1 and ROI 2) in the hind limbs. (D) CLSM imaging of a dorsal skinfold chamber model. (E) In vivo monitoring of temporal distribution of GSHPTcy5CPT in tumor blood vessels. (F) 3D reconstruction PA images of tumor bearing mice injected with molecule 1, SiO2 nanoparticles and liposome labeled by Cy. The tumor tissue was divided into five parts with identical radius intervals. Scale bar: 2 mm. Note: SQ: squaraine dye; NIR-II: near-infrared light II; CLSM: confocal laser scanning microscopy; GSHPTcy5CPT: a GGT-triggered charge-reversal dendrimer-CPT conjugate marked with cy5. (Fig. 4A was created with BioRender.com. Fig. 4B was reprinted with permission from Ref. [51]. Copyright 2023 American Chemical Society. Fig. 4C was reprinted with permission from Ref. [53]. Copyright 2020 American Chemical Society. Fig. 4D and E were reprinted with permission from Ref. [55]. Copyright 2020 American Chemical Society. Fig. 4F was reprinted with permission from Ref. [59]. Copyright 2019 Springer Nature.).