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(A) 3D Culture of A549 cells in WBPU scaffolds suggested a 3D pattern of colony-like growth of tumor cells on the scaffolds, with spheroid growth of tumor cells on PU4 scaffolds, scale bars 100 μm for low-magnification field and 20 μm for high-magnification field. (B) The establishment of 3D spheroids on PU4 scaffolds after 48 h of cultivation, scale bar 20 μm. (C) Cell proliferation in 2D- and 3D-cultured A549 cells measured by MTT (n = 3). ∗P < 0.05; ns: P > 0.05.
